Publications by Radhika Subramanian
2025 Publications
Collaborative role of two distinct cilium-specific cytoskeletal systems in driving Hedgehog-responsive transcription factor trafficking
Ku P, Sreeja JS, Chadha A, Williams DS, Engelke MF, Radhika Subramanian*. [2025] Sci. Adv.11,eadt5439(2025).
- Preprint (2024): https://www.biorxiv.org/content/10.1101/2024.09.26.615198v2
Non-canonical CDK6 activity promotes cilia disassembly by suppressing axoneme polyglutamylation
Kai He, Xiaobo Sun, Chuan Chen, San Luc, Jielu Hao Robichaud, Yingyi Zhang,Yan Huang, Biyun Ji, Pei-I Ku, Radhika Subramanian, Kun Ling, Jinghua Hu. [2025] J Cell Biol (2025) 224 (2): e202405170.
- Preprint (2023): https://www.biorxiv.org/content/10.1101/2023.06.22.546046v1
2024 Publications
GRK2 kinases in the primary cilium initiate SMOOTHENED-PKA signaling in the Hedgehog cascade
Madison F. Walker*, Jingyi Zhang*, William Steiner*, Pei-I Ku*, Ju-Fen Zhu,Zachary, Michaelson, Yu-Chen, Yen, Annabel Lee, Alyssa B. Long, Mattie J. Casey, Abhishek Poddar, Isaac B. Nelson, Corvin D. Arveseth, Falko Nagel, Ryan Clough, Sarah LaPotin, Kristen M. Kwan, Stefan Schulz, Rodney A. Stewart, John J. G. Tesmer, Tamara Caspary, Radhika Subramanian*, Xuecai Ge*, Benjamin R. Myers*. [2024] PLoS Biol. 2024 Aug 13;22(8):e3002685.
- Preprint (2023): https://www.biorxiv.org/content/10.1101/2023.05.10.540226v3
Torques within and outside the human spindle balance twist at anaphase
Torques within and outside the human spindle balance twist at anaphase
Lila Neahring, Nathan H. Cho, Yifei He, Gaoxiang Liu, Jonathan Fernandes, Caleb J. Rux, Konstantinos Nakos, Radhika Subramanian, Srigokul Upadhyayula, Ahmet Yildiz, Sophie Dumont. [2024] J Cell Biol (2024) 223 (9): e202312046.
- Preprint (2023): https://www.biorxiv.org/content/10.1101/2023.12.10.570990v1
Modularity of PRC1 Composition and Chromatin Interaction define Condensate Properties
Stefan Niekamp, Sharon K. Marr, Theresa A. Oei, Radhika Subramanian*, Robert E. Kingston*. [2024] Volume 84, Issue 9, 2 May 2024, Pages 1651-1666.e12
- Preprint (2023): https://www.biorxiv.org/content/10.1101/2023.10.26.564217v2
Architecture and flexibility of native kinetochores revealed by structural studies utilizing a thermophilic yeast
Daniel J. Barrero, Sithara S. Wijeratne, Xiaowei Zhao, Grace F. Cunningham, Rui Yan, Christian R. Nelson, Yasuhiro Arimura, Hironori Funabiki, Charles L. Asbury, Zhiheng Yu, Radhika Subramanian, Sue Biggins. [2024] Curr Biol. 2024 Sep 9;34(17):3881-3893.e5.
- Preprint (2023): https://www.biorxiv.org/content/10.1101/2023.12.10.570990v1
Cytoskeleton crosstalk: Casting stable actin bundles with dynamic microtubule molds
2023 Publications
Real-time visualization of microtubule and protofilament-scale dynamics in multi-microtubule arrays by atomic force microscopy
Wijeratne, S. S*., & Subramanian, R*. [2023] Current Protocols, 3, e779.
Interplay of self-organization of microtubule asters and crosslinking protein condensates
Sahu S, Chauhan P, Lumen E, Moody K, Peddireddy K, Mani N, Subramanian R, Robertson-Anderson R, Wolfe AJ, Ross JL. [2023] PNAS Nexus, Volume 2, Issue 7, July 2023, pgad231
- Preprint (2022): https://www.biorxiv.org/content/10.1101/2022.10.23.513406v1
2022 Publications
Active microphase separation in mixtures of microtubules and tip-accumulating molecular motors
Lemma B, Mitchell NP, Subramanian R, Needleman DJ, Dogic Z. [2022] Physical Review X 12, 031006.
Motor guidance by long-range communication through the microtubule highway
Wijeratne SS, Fiorenza SA, Subramanian R*, Betterton MD*. [2022] *co-corresponding/co-senior author. Proc Natl Acad Sci USA. 2022 July 7;119 (28) e2120193119.
- Preprint (2020): https://www.biorxiv.org/content/10.1101/2020.12.23.424221v1
Cytoskeletal regulation of a transcription factor by DNA mimicry via coiled-coil interactions
Haque F, Freniere C, Ye Q, Mani N, Wilson-Kubalek LM, Ku Pi-I, Milligan RA, Subramanian, R*. [2022] Nat Cell Biol 24, 1088–1098 (2022).
- News and Views: Kumar, D., Reiter, J.F. A kinesin mimics DNA. Nat Cell Biol (2022)
- Preprint (2020): https://www.biorxiv.org/content/10.1101/2021.09.30.462597v1
Atomic Force Microscopy reveals distinct protofilament-scale structural dynamics in depolymerizing microtubule arrays
Wijeratne SS, Marchan MF, Tresback JS, Subramanian R*. [2022] Proc Natl Acad Sci USA. 2022 Feb 1;119(5):e2115708119.
- Preprint (2020): https://www.biorxiv.org/content/10.1101/2020.12.22.423986v1
Engineering stability, longevity, and miscibility of microtubule-based active fluids
Chandrakar P, Berezney J, Lemma B, Hishamunda B, Berry A, Wu K-T, Subramanian R, Chung J, Needleman DJ, Gelles J, Dogic, Z. [2022] Soft Matter, 2022,18, 1825-1835
- Preprint (2018): https://arxiv.org/abs/1811.05026
Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
Mani N, Marchan M F, Subramanian R*. [2022] J. Vis. Exp. (180), e63377
2021 Publications
The tail wags the tubulin
Mani N and Subramanian R*. [2021] Developmental Cell, Volume 56, Issue 14, 26 July 2021, pages 2007-2009. (preview)
Micron-scale geometrical features of microtubules as regulators of microtubule organization
Mani N, Wijeratne S and Subramanian R*. [2021] eLife, Jun 11; 10:e63880.
doi: 10.7554/eLife.63880.
Differential Regulation of Single Microtubules and Cross-linked Bundles by a Minimal Three-Protein Module
Mani N, Jiang S, Neary AE, Wijeratne S and Subramanian R*. [2021] Nat Chem Biol 17, 964–974 (2021)
- Preprint (2020): https://www.biorxiv.org/content/10.1101/2020.03.05.979864v1
2019 Publications
Mitotic spindle: From living and synthetic systems to theory
Simunic J*, Subramanian R*. [2019] Journal of Cell Science. 2019 Sep 2;132(17). Meeting report.
Interplay between the Kinesin and Tubulin Mechanochemical Cycles Underlies Microtubule Tip Tracking by the Non-motile Ciliary
Jiang S, Mani N, Wilson-Kubalek EM, Ku PI, Milligan RA, Subramanian R*.
Kinesin Kif7. [2019] Dev. Cell 2019 Jun 03; 49(5):711-730.e8
2018 Publications
Composition, Structure, and Function of the Eukaryotic Flagellum Distal Tip
Croft J, Zabeo D, Subramanian R and Hoog J. [2018] Essays in Biochemistry. 2018 Nov 20 pii: EBC20180032
Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A
Wijeratne S, Subramanian R*. [2018] Elife 2018 10 24; 7
2015 Publications
Niche-Based Screening in Multiple Myeloma Identifies a Kinesin-5 Inhibitor with Improved Selectivity over Hematopoietic Progenitors
Chattopadhyay S, Stewart AL, Mukherjee S, Huang C, Hartwell KA, Miller PG, Subramanian R, Carmody LC, Yusuf RZ, Sykes DB, Paulk J, Vetere A, Vallet S, Santo L, Cirstea DD, Hideshima T, Majireck MM, Hussain MM, Singh S, Quiroz R, Iaconelli J, Karmacharya R, Tolliday NJ, Clemons PA, Moore MAS, Stern AM, Shamji AF, Ebert BL, Golub TR, Raje NS, Scadden DT, Schreiber SL. [2015] Cell Rep 2015 Feb 10; 10(5):755-770
2014 Publications
The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment
He M, Subramanian R, Bangs F, Omelchenko T, Liem KF, Kapoor TM, Anderson KV. [2014] Nat. Cell Biol. 2014 Jul; 16(7):663-72
2013 Publications
Slipping past the spindle assembly checkpoint
Subramanian R, Kapoor TM. [2013] Nat. Cell Biol. 2013 Nov; 15(11):1261-3
Marking and measuring single microtubules by PRC1 and kinesin-4
Subramanian R, Ti SC, Tan L, Darst SA, Kapoor TM. [2013] Cell 2013 Jul 18; 154(2):377-90
2012 Publications
Building complexity: insights into self-organized assembly of microtubule-based architectures
Subramanian R, Kapoor TM. [2012] Dev. Cell 2012 Nov 13; 23(5):874-85
2010 Publications
Insights into antiparallel microtubule crosslinking by PRC1, a conserved nonmotor microtubule binding protein
Subramanian R, Wilson-Kubalek EM, Arthur CP, Bick MJ, Campbell EA, Darst SA, Milligan RA, Kapoor TM. [2010] Cell 2010 Aug 6; 142(3):433-43
2009 Publications
Meiotic spindle self-organization: one plus one equals only one
Subramanian R, Kapoor T. [2009] Curr. Biol. 2009 Apr 14; 19(7):R290-2
2007 Publications
Two distinct modes of processive kinesin movement in mixtures of ATP and AMP-PNP
Subramanian R, Gelles J. [2007] J. Gen. Physiol. 2007 Nov; 130(5):445-55