Publications by the Subramanian lab| Subramanian Lab

2024 Publications

Collaborative role of two distinct cilium-specific cytoskeletal systems in driving Hedgehog-responsive transcription factor trafficking

View ORCID Profile
Pei-I Ku*, Jamuna S Sreeja*, Abhishek Chadha, David S Williams, Martin F Engelke, Radhika Subramanian*

https://www.biorxiv.org/content/10.1101/2024.09.26.615198v2

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GRK2 kinases in the primary cilium initiate SMOOTHENED-PKA signaling in the Hedgehog cascade

Madison F. Walker*, Jingyi Zhang*, William Steiner*, Pei-I Ku*, Ju-Fen Zhu,Zachary, Michaelson, Yu-Chen, Yen, Annabel Lee, Alyssa B. Long, Mattie J. Casey, Abhishek Poddar, Isaac B. Nelson, Corvin D. Arveseth, Falko Nagel, Ryan Clough, Sarah LaPotin, Kristen M. Kwan, Stefan Schulz, Rodney A. Stewart, John J. G. Tesmer, Tamara Caspary, Radhika Subramanian*, Xuecai Ge*, Benjamin R. Myers*

https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3002685

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Torques within and outside the human spindle balance twist at anaphase

Lila Neahring, Nathan H. Cho, Yifei He, Gaoxiang Liu, Jonathan Fernandes, Caleb J. Rux, Konstantinos Nakos, Radhika Subramanian, Srigokul Upadhyayula, Ahmet Yildiz, Sophie Dumont

https://rupress.org/jcb/article-abstract/223/9/e202312046/276808/Torques-within-and-outside-the-human-spindle?redirectedFrom=fulltext

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Modularity of PRC1 Composition and Chromatin Interaction define Condensate Properties

Stefan Niekamp, Sharon K. Marr, Theresa A. Oei, Radhika Subramanian*, Robert E. Kingston*
https://www.sciencedirect.com/science/article/pii/S1097276524001813?via%3Dihub

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Architecture and flexibility of native kinetochores revealed by structural studies utilizing a thermophilic yeast

View ORCID Profile Daniel J. Barrero, Sithara S. Wijeratne, Xiaowei Zhao, Grace F. Cunningham, Rui Yan, Christian R. Nelson, Yasuhiro Arimura, Hironori Funabiki, Charles L. Asbury, Zhiheng Yu, Radhika Subramanian, View ORCID Profile Sue Biggins
doi: https://doi.org/10.1101/2024.02.28.582571

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Cytoskeleton crosstalk: Casting stable actin bundles with dynamic microtubule molds

Farah Haque and Radhika Subramanian* Current Biology Volume 34, Issue 2, 22 January 2024, Pages R72-R74; https://www.sciencedirect.com/science/article/abs/pii/S0960982223016846?via%3Dihu

2023 Publications

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Torques within and outside the human spindle balance twist at anaphase

Lila Neahring, Yifei He, Nathan H. Cho, Gaoxiang Liu, Jonathan Fernandes, Caleb J. Rux, Konstantinos Nakos, Radhika Subramanian, Srigokul Upadhyayula, Ahmet Yildiz, Sophie Dumont
bioRxiv 2023.12.10.570990; doi: https://doi.org/10.1101/2023.12.10.570990

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Modularity of PRC1 Composition and Chromatin Interaction define Condensate Properties

Stefan Niekamp, Sharon K. Marr, Theresa A. Oei, Radhika Subramanian*, Robert E. Kingston*
bioRxiv 2023.10.26.564217; doi: https://doi.org/10.1101/2023.10.26.564217

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Real-time visualization of microtubule and protofilament-scale dynamics in multi-microtubule arrays by atomic force microscopy

Wijeratne, S. S*., & Subramanian, R*. (2023). Current Protocols, 3, e779. https://doi.org/10.1002/cpz1.779; *co-corresponding authors

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Spatially controlled microtubule nucleation and organization from crosslinker MAP65 condensates

Sahu S, Chauhan P, Lumen E, Moody K, Peddireddy K, Mani N, Subramanian R, Robertson-Anderson R, Wolfe AJ, Ross JL. doi: https://doi.org/10.1101/2022.10.23.513406

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A Ciliary SMOOTHENED-GRK2-PKA Signaling Pathway Initiates Hedgehog Signal Transduction

Madison F Walker, Jingyi Zhang, William Steiner, Pei-I Ku, Ju-Fen Zhu, Zachary Michaelson, Yu-Chen Yen, Alyssa B. Long, Mattie J. Casey, Abhishek Poddar, Isaac B. Nelson, Corvin D. Arveseth, Falko Nagel, Ryan Clough, Sarah LaPotin, Kristen M. Kwan, Stefan Schulz, Rodney A. Stewart, John J. G. Tesmer, Tamara Caspary, Radhika Subramanian*, Xuecai Ge*, Benjamin R. Myers*.
View ORCID Profile
doi: https://doi.org/10.1101/2023.05.10.540226 *co-corresponding authors

2022 Publications

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Active microphase separation in mixtures of microtubules and tip-accumulating molecular motors

Lemma B, Mitchell NP, Subramanian R, Needleman DJ, Dogic Z. (2022) Physical Review X 12, 031006. https://journals.aps.org/prx/abstract/10.1103/PhysRevX.12.031006

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Motor guidance by long-range communication through the microtubule highway

Wijeratne SS, Fiorenza SA, Subramanian R*, Betterton MD*. (2022) *co-corresponding/co-senior author. Proc Natl Acad Sci USA. 2022 July 7;119 (28) e2120193119. https://doi.org/10.1073/pnas.2120193119

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Cytoskeletal regulation of a transcription factor by DNA mimicry via coiled-coil interactions

Haque F, Freniere C, Ye Q, Mani N, Wilson-Kubalek LM, Ku Pi-I, Milligan RA, Subramanian, R. (2022) Nature Cell Biology. https://doi.org/10.1038/s41556-022-00935-7
(News and Views: Kumar, D., Reiter, J.F. A kinesin mimics DNA. Nat Cell Biol (2022) https://doi.org/10.1038/s41556-022-00958-0)

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Atomic Force Microscopy reveals distinct protofilament-scale structural dynamics in depolymerizing microtubule arrays

Wijeratne SS, Marchan MF, Tresback JS, Subramanian R. (2022) Proc Natl Acad Sci USA. 2022 Feb 1;119(5):e2115708119. https://doi.org/10.1073/pnas.2115708119

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Engineering stability, longevity, and miscibility of microtubule-based active fluids

Chandrakar P, Berezney J, Lemma B, Hishamunda B, Berry A, Wu K-T, Subramanian R, Chung J, Needleman DJ, Gelles J, Dogic, Z. (2022) Soft Matter. doi: 10.1039/D1SM01289D.

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Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Mani N, Marchan M F, Subramanian R. (2022) J. Vis. Exp. (180), e63377, https://dx.doi.org/10.3791/63377.

2021 Publications

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Cytoskeletal regulation of a transcription factor by DNA mimicry

Farah Haque, Christian Freniere, Qiong Ye, Nandini Mani, Elizabeth M. Wilson-Kubalek, Pei-I Ku, Ronald A. Milligan, Radhika Subramanian. bioRxiv, 2021 October 01.
https://www.biorxiv.org/content/10.1101/2021.09.30.462597v1

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The tail wags the tubulin

Mani N and Subramanian R (2021). Developmental Cell, Volume 56, Issue 14, 26 July 2021, pages 2007-2009. (preview)
doi: https://doi.org/10.1016/j.devcel.2021.06.018

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Micron-scale geometrical features of microtubules as regulators of microtubule organization

Mani N, Wijeratne S and Subramanian R (2021). eLife, Jun 11; 10:e63880.
doi: 10.7554/eLife.63880.

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Differential Regulation of Single Microtubules and Cross-linked Bundles by a Minimal Three-Protein Module

Mani N, Jiang S, Neary AE, Wijeratne S and Subramanian R. (2021) Nature Chemical Biology. https://doi.org/10.1038/s41589-021-00800-y

2020 Publications

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Motor guidance by long-range communication through the microtubule highway

Sithara S. Wijeratne, Shane A. Fiorenza, Radhika Subramanian, Meredith D. Betterton
bioRxiv, 2020 December 23

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Distinct Protofilament-scale Dynamics Define the Differential Remodeling of Microtubule Arrays

S. S. Wijeratne, M. Marchan, J. S. Tresback, R. Subramanian
bioRxiv, 2020 December 22

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Differential Regulation of Single Microtubules and Cross-linked Bundles by a Minimal Three-Protein Module

Mani N, Jiang S, Subramanian R.
bioRxiv, 2020 March 5

2019 Publications

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Mitotic spindle: From living and synthetic systems to theory

Simunic J, Subramanian R.
Journal of Cell Science. 2019 Sep 2;132(17). Meeting report. (link: https://jcs-biologists-org.ezp-prod1.hul.harvard.edu/content/132/17/jcs237602.long)

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Interplay between the Kinesin and Tubulin Mechanochemical Cycles Underlies Microtubule Tip Tracking by the Non-motile Ciliary

Jiang S, Mani N, Wilson-Kubalek EM, Ku PI, Milligan RA, Subramanian R.
Kinesin Kif7. Dev. Cell 2019 Jun 03; 49(5):711-730.e8

2018 Publications

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Composition, Structure, and Function of the Eukaryotic Flagellum Distal Tip

Croft J, Zabeo D, Subramanian R and Hoog J.
2018 Essays in Biochemistry. 2018 Nov 20 pii: EBC20180032

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Microtubule-based active fluids with improved lifetime, temporal stability and miscibility with passive soft materials

Chandrakar P, Berezney J, Lemma B, Hishamunda B, Berry A, Wu K, Subramanian R, Chung J, Needleman D, Gelles J and Dogic Z.
arXiv:1811.05026v1 [cond-mat.soft]
2018 November 12

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Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A

Wijeratne S, Subramanian R.
Elife 2018 10 24; 7

2015 Publications

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Niche-Based Screening in Multiple Myeloma Identifies a Kinesin-5 Inhibitor with Improved Selectivity over Hematopoietic Progenitors

Chattopadhyay S, Stewart AL, Mukherjee S, Huang C, Hartwell KA, Miller PG, Subramanian R, Carmody LC, Yusuf RZ, Sykes DB, Paulk J, Vetere A, Vallet S, Santo L, Cirstea DD, Hideshima T, Majireck MM, Hussain MM, Singh S, Quiroz R, Iaconelli J, Karmacharya R, Tolliday NJ, Clemons PA, Moore MAS, Stern AM, Shamji AF, Ebert BL, Golub TR, Raje NS, Scadden DT, Schreiber SL.
Cell Rep 2015 Feb 10; 10(5):755-770